anti phos akt  (Santa Cruz Biotechnology)

Journal: Drug Design, Development and Therapy

Article Title: Combination of Sodium Cantharidinate with Cisplatin Synergistically Hampers Growth of Cervical Cancer

doi: 10.2147/DDDT.S282777

Figure Lengend Snippet: SC inhibits the growth as well as drug resistance of CC cells in vivo. ( A ) process diagram; ( B ) growth curves of tumors; ( C ) weight of tumors; ( D – G ) immunohistochemical staining of the number of positive cells for KI67 ( D ), PTPN1 ( E ), phos-PI3K ( F ), and phos-AKT1 ( G ) in xenograft tumors; ( H ), the proportion of apoptotic cells in tumors determined by TUNEL staining. The experiments were repeated at least three times. N = 6. The data are expressed as the means ± SD of three experiments. Statistical analysis was performed utilizing the one-way (panel C – H ) or two-way ANOVA (panel B ) test combined with Tukey’s test. ** p < 0.01 vs mice injected with Caski-1 + PBS; ## p < 0.01 vs mice injected with Caski-1/R + PBS.

Article Snippet: The membranes were sealed with 5% skim milk for 90 min at ambient temperature, followed by incubation with primary antibodies (1:1000 dilution) to PTPN1 (MABS197, Millipore), phos-PI3K (ab182651, Abcam), and phos-AKT1 (#bs-1448R, Bioss Biotech, Beijing, China) at 4°C overnight and with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (1:10,000 dilution; Proteintech, Chicago, IL, USA) for 60 min at ambient temperature.

Techniques: In Vivo, Immunohistochemical staining, Staining, TUNEL Assay, Injection